Table of Larval Stages

 

Larval Stages of Drosophila melanogaster
(at 25°C)

Time After Fertilization
Hours Days Developmental event
24 1 Hatching from egg; first larval instar begins
49 2 First molt; second instar begins
72 3 Second molt; third instar begins
120 5 Puparium formation; puparium white
122 5.1 Puparium fully colored
124 5.2 “Prepupal” molt
132 5.5 Pupation; cephalic complex, wings, legs everted
169 7 Eye pigmentation begins
189 7.9 Bristle pigmentation begins
216 9 Adult ready to emerge from pupa case
Source: After Doane, 1967. Drosophila. In Methods in Developmental Biology, F. H. Wilt and N. K. Wessells (eds.). Thomas Y. Crowell Co., New York, pp. 219–244.